By Timothy Richard Parsons

An creation to the quantitative research of seawater, describing intimately organic and chemical options, that are thought of to be among these pretty much utilized by organic oceanographers. The guide presents entire directions for the addition of reagents and calculation of effects with regard fabric for every strategy in order that the unique texts should be consulted if worthwhile. quite often, the options require at the very least past specialist education and techniques desiring very pricey apparatus were shunned.

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Store both the concentrated and diluted vitamin solutions at — 20°C in glass bottles; the diluted vitamin solution is conveniently dispensed into 10 ml aliquots in screw-cap test tubes for use with each batch of samples. Nutrients 3. 5% saline are either purchased or made up and sterilized by autoclaving. 4. 05 ml of nutrient solution 2(iii). Additions should be made aseptically and the flasks stoppered with cotton wool in cheesecloth. 05 ml of vitamin B i 2 solution (Section G); this flask is the stock solution and is used to maintain the culture.

6. 2 ml of M B T H reagent into the sample solution. Allow the tightly-capped tubes to stand for 3 min in a boiling water bath. Then cool the sample to room temperature with tap water. 7. 2 ml of ferric chloride solution into the sample and let stand for 30 min at room temperature in the dark. 8. Add 1 ml of acetone and mix. 9. 8:1 of watenacetone mixture at a wavelength of 635 nm. 10. Correct the measured extinction by subtracting the analytical blank (see Section G). Calculate the total monosaccharide concentration in water samples in /imoles/1 from the expression, ^mole/1 = (Es — Eh)xF, where Es is the mean extinction of triplicate analyses of water samples, Eh is the mean extinction of duplicate analyses of analytical blanks and F i s determined as described below.

7N HC1. Remove the cap of the test tube and leave for 10 min at room temperature to allow hydrogen gas to evolve. The p H of the solution should be optimal at ca. 5 for the next step (Note d). 3. 1 ml of periodic acid solution into the sample solution and let stand for 10 min at room temperature in the dark. In this reaction, two hydroxyl groups of alditol are oxidized to aldehyde groups. 4. 1 ml of sodium arsenite solution into the sample solution in order to stop the oxidation reaction. Let stand for at least 10 min in the dark (Note e).

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